This is a test that measures the level of chloride in sweat.

A low-level electric current is applied to the test area. The positive electrode is covered by gauze and saturated with pilocarpine (a drug that stimulates sweating). The negative electrode is covered with gauze and saturated with bicarbonate solution.

A current is applied for 5 to 12 minutes, and a paper disk is then placed over the test site and covered with paraffin to obtain an airtight seal. After 1 hour, the disk is transferred to a weighing jar, then put in water to dissolve the salts. This solution is then analyzed for chloride.

No special preparation is necessary. Make sure the center where the test is being performed is a cystic fibrosis testing center.

The test does not cause any discomfort.

Children with cystic fibrosis have increased sodium and chloride concentrations in their sweat. The test may be performed on infants to determine if early therapy is needed.

Normal values are as follows:

• Sodium:
  • Normal: less than 70 mEq/L
  • Abnormal: greater than 90 mEq/L
  • Equivocal: 70 to 90 mEq/L
• Chloride:
  • Normal: less than 50 mEq/L
  • Abnormal: greater than 60 mEq/L
  • Equivocal: 50 to 60 mEq/L

An abnormal test may indicate the presence of cystic fibrosis.