Complement C3 is a blood test that measures the activity of a certain protein that is part of the complement system. The complement system is a group of proteins that move freely through your bloodstream. The proteins work with your immune system and play a role in the development of inflammation.

There are nine major complement proteins. They are labeled C1 through C9. This test measures C3.

See also:

• Complement
• C1 inhibiting factor
• Complement component 4 (C4)
• CH50
• CH100

Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.

Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm.

Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.

There is no special preparation needed.

When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.

C3 and C4 are the most commonly measured complement components.

A complement test may be used to monitor patients with an autoimmune disorder and to see if treatment for their condition is working. For example, patients with active lupus erythematosus may have lower-than-normal levels of the complement proteins C3 and C4.

Complement activity varies throughout the body. For example, in patients with rheumatoid arthritis, complement activity in the blood may be normal or higher-than-normal, but much lower-than-normal in the joint fluid.

Additional conditions under which the test may be performed:

• Gram negative septicemia
• Shock
• Fungal infections
• Parasitic infections such as malaria
• Paroxysmal nocturnal hemoglobinuria (PNH)

The normal range is 75-135 mg/dl (milligrams per deciliter).

Increased complement activity may be seen in:

• Cancer
• Ulcerative colitis

• Hereditary angioedema
• Bacterial infections (especially Neisseria)
• Cirrhosis
• Glomerulonephritis
• Hepatitis
• Lupus nephritis
• Malnutrition
• Kidney transplant rejection
• Systemic lupus erythematosus

• Excessive bleeding
• Fainting or feeling light-headed
• Hematoma (blood accumulating under the skin)
• Infection (a slight risk any time the skin is broken)
• Multiple punctures to locate veins

The "complement cascade" is a series of reactions that take place in the blood. The cascade activates the complement proteins. The result is an attack unit that creates holes in the membrane of bacteria, killing them. C3 attaches to and kills bacteria directly.