2.3 DDBJ Example Flat File
Example 2-2 contains
a sample sequence entry from DDBJ. This entry contains terms from the
DDBJ Field Definitions and the DDBJ/EMBL/GenBank Feature Table,
discussed later in this chapter.
Example 2-2. Sample DDBJ entry
LOCUS HSCDK2MR 1476 bp RNA linear HUM 15-JAN-1992
DEFINITION H.sapiens CDK2 mRNA.
ACCESSION X61622
VERSION X61622.1
KEYWORDS CDK2 gene; cell cycle regulation protein; cyclin A binding; protein
kinase.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1476)
AUTHORS Elledge,S.J. and Spottswood,M.R.
TITLE A new human p34 protein kinase, CDK2, identified by complementation
of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of
Xenopus Eg1
JOURNAL EMBO J. 10, 2653-2659(1991).
MEDLINE 91330891
REFERENCE 2 (bases 1 to 1476)
AUTHORS Elledge,S.J.
JOURNAL Submitted (28-NOV-1991) to the EMBL/GenBank/DDBJ databases. S.J.
Elledge, Dept. of Biochemistry, Baylor College of Medicine, 1 Baylor
Place, Houston, TX 77030, USA
FEATURES Location/Qualifiers
source 1..1476
/db_xref="taxon:9606"
/organism="Homo sapiens"
/cell_line="EBV transformed Human peripheral lymphocyte
(B-cell)"
/clone_lib="lambda YES-R cDNA library"
/clone="pSE1000"
CDS 1..897
/db_xref="SWISS-PROT:P24941"
/note="cell division kinase. CDC2 homolog"
/gene="CDK2"
/function="protein kinase"
/protein_id="CAA43807.1"
/translation="MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVP
STAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLI
KSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEV
VTLWYRAPEILLGSKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTP
DEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAAL
AHPFFQDVTKPVPHLRL"
BASE COUNT 368 a 372 c 351 g 385 t
ORIGIN
1 atggagaact tccaaaaggt ggaaaagatc ggagagggca cgtacggagt tgtgtacaaa
61 gccagaaaca agttgacggg agaggtggtg gcgcttaaga aaatccgcct ggacactgag
121 actgagggtg tgcccagtac tgccatccga gagatctctc tgcttaagga gcttaaccat
181 cctaatattg tcaagctgct ggatgtcatt cacacagaaa ataaactcta cctggttttt
241 gaatttctgc accaagatct caagaaattc atggatgcct ctgctctcac tggcattcct
301 cttcccctca tcaagagcta tctgttccag ctgctccagg gcctagcttt ctgccattct
361 catcgggtcc tccaccgaga ccttaaacct cagaatctgc ttattaacac agagggggcc
421 atcaagctag cagactttgg actagccaga gcttttggag tccctgttcg tacttacacc
481 catgaggtgg tgaccctgtg gtaccgagct cctgaaatcc tcctgggctc gaaatattat
541 tccacagctg tggacatctg gagcctgggc tgcatctttg ctgagatggt gactcgccgg
601 gccctgttcc ctggagattc tgagattgac cagctcttcc ggatctttcg gactctgggg
661 accccagatg aggtggtgtg gccaggagtt acttctatgc ctgattacaa gccaagtttc
721 cccaagtggg cccggcaaga ttttagtaaa gttgtacctc ccctggatga agatggacgg
781 agcttgttat cgcaaatgct gcactacgac cctaacaagc ggatttcggc caaggcagcc
841 ctggctcacc ctttcttcca ggatgtgacc aagccagtac cccatcttcg actctgatag
901 ccttcttgaa gcccccgacc ctaatcggct caccctctcc tccagtgtgg gcttgaccag
961 cttggccttg ggctatttgg actcaggtgg gccctctgaa cttgccttaa acactcacct
1021 tctagtctta accagccaac tctgggaata caggggtgaa aggggggaac cagtgaaaat
1081 gaaaggaagt ttcagtatta gatgcactta agttagcctc caccaccctt tcccccttct
1141 cttagttatt gctgaagagg gttggtataa aaataatttt aaaaaagcct tcctacacgt
1201 tagatttgcc gtaccaatct ctgaatgccc cataattatt atttccagtg tttgggatga
1261 ccaggatccc aagcctcctg ctgccacaat gtttataaag gccaaatgat agcgggggct
1321 aagttggtgc ttttgagaat taagtaaaac aaaaccactg ggaggagtct attttaaaga
1381 attcggttaa aaaatagatc caatcagttt ataccctagt tagtgttttc ctcacctaat
1441 aggctgggag actgaagact cagcccgggt gggggt
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