2.5 EMBL Example Flat File

Example 2-3 contains a sample sequence entry from EMBL. This entry contains terms from the EMBL Field Definitions and the DDBJ/EMBL/GenBank Feature Table, discussed later in this chapter.

Example 2-3. Sample EMBL entry

ID   HSCDK2MR   standard; RNA; HUM; 1476 BP.
XX
AC   X61622;
XX
SV   X61622.1
XX
DT   15-JAN-1992 (Rel. 30, Created)
DT   15-JAN-1992 (Rel. 30, Last updated, Version 1)
XX
DE   H.sapiens CDK2 mRNA
XX
KW   CDK2 gene; cell cycle regulation protein; cyclin A binding; protein kinase.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Primates; Catarrhini; Hominidae; Homo.
XX
RN   [1]
RP   1-1476
RX   MEDLINE; 91330891.
RA   Elledge S.J., Spottswood M.R.;
RT   "A new human p34 protein kinase, CDK2, identified by complementation of a
RT   cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1";
RL   EMBO J. 10:2653-2659(1991).
XX
RN   [2]
RP   1-1476
RA   Elledge S.J.;
RT   ;
RL   Submitted (28-NOV-1991) to the EMBL/GenBank/DDBJ databases.
RL   S.J. Elledge, Dept. of Biochemistry, Baylor College of Medicine, 1 Baylor
RL   Place, Houston, TX 77030, USA
XX
DR   GDB; 128984; CDK2.
DR   SWISS-PROT; P24941; CDK2_HUMAN.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1476
FT                   /db_xref="taxon:9606"
FT                   /organism="Homo sapiens"
FT                   /cell_line="EBV transformed Human peripheral lymphocyte
FT                   (B-cell)"
FT                   /clone_lib="lambda YES-R cDNA library"
FT                   /clone="pSE1000"
FT   CDS             1..897
FT                   /db_xref="SWISS-PROT:P24941"
FT                   /note="cell division kinase. CDC2 homolog"
FT                   /gene="CDK2"
FT                   /function="protein kinase"
FT                   /protein_id="CAA43807.1"
FT                   /translation="MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVP
FT                   STAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLI
FT                   KSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEV
FT                   VTLWYRAPEILLGSKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTP
FT                   DEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAAL
FT                   AHPFFQDVTKPVPHLRL"
XX
SQ   Sequence 1476 BP; 368 A; 372 C; 351 G; 385 T; 0 other;
     atggagaact tccaaaaggt ggaaaagatc ggagagggca cgtacggagt tgtgtacaaa        60
     gccagaaaca agttgacggg agaggtggtg gcgcttaaga aaatccgcct ggacactgag       120
     actgagggtg tgcccagtac tgccatccga gagatctctc tgcttaagga gcttaaccat       180
     cctaatattg tcaagctgct ggatgtcatt cacacagaaa ataaactcta cctggttttt       240
     gaatttctgc accaagatct caagaaattc atggatgcct ctgctctcac tggcattcct       300
     cttcccctca tcaagagcta tctgttccag ctgctccagg gcctagcttt ctgccattct       360
     catcgggtcc tccaccgaga ccttaaacct cagaatctgc ttattaacac agagggggcc       420
     atcaagctag cagactttgg actagccaga gcttttggag tccctgttcg tacttacacc       480
     catgaggtgg tgaccctgtg gtaccgagct cctgaaatcc tcctgggctc gaaatattat       540
     tccacagctg tggacatctg gagcctgggc tgcatctttg ctgagatggt gactcgccgg       600
     gccctgttcc ctggagattc tgagattgac cagctcttcc ggatctttcg gactctgggg       660
     accccagatg aggtggtgtg gccaggagtt acttctatgc ctgattacaa gccaagtttc       720
     cccaagtggg cccggcaaga ttttagtaaa gttgtacctc ccctggatga agatggacgg       780
     agcttgttat cgcaaatgct gcactacgac cctaacaagc ggatttcggc caaggcagcc       840
     ctggctcacc ctttcttcca ggatgtgacc aagccagtac cccatcttcg actctgatag       900
     ccttcttgaa gcccccgacc ctaatcggct caccctctcc tccagtgtgg gcttgaccag       960
     cttggccttg ggctatttgg actcaggtgg gccctctgaa cttgccttaa acactcacct      1020
     tctagtctta accagccaac tctgggaata caggggtgaa aggggggaac cagtgaaaat      1080
     gaaaggaagt ttcagtatta gatgcactta agttagcctc caccaccctt tcccccttct      1140
     cttagttatt gctgaagagg gttggtataa aaataatttt aaaaaagcct tcctacacgt      1200
     tagatttgcc gtaccaatct ctgaatgccc cataattatt atttccagtg tttgggatga      1260
     ccaggatccc aagcctcctg ctgccacaat gtttataaag gccaaatgat agcgggggct      1320
     aagttggtgc ttttgagaat taagtaaaac aaaaccactg ggaggagtct attttaaaga      1380
     attcggttaa aaaatagatc caatcagttt ataccctagt tagtgttttc ctcacctaat      1440
     aggctgggag actgaagact cagcccgggt gggggt                                1476
//