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SIMPLE See also:MICROSCOPE Position and See also:Size of the See also:Image.—A See also:person with normal See also:vision can see See also:objects distinctly at a distance varying from ten. inches to a very See also:great distance. Objects at different distances, however, are not seen distinctly simultaneously, but in See also:succession. This is effected by the See also:power of See also:accommodation of the See also:eye, which can so alter the See also:focal length of its crystalline See also:lens that images of objects at different distances can be produced rapidly and distinctly one after another upon the retina. The See also:angle under which the See also:object appears depends upon the distance and size of the object, or, in other words, the size of the image on the retina is determined by the distance and the dimensions of the object. The ratio between the real size of the object y (fig. 1) 1 and the distance 1, which is equal to the tangent of the visual angle w, is termed the " apparent size " of the object. From the figure, which represents vision with a motionless eye, it is seen that the apparent size increases as the object under observation is approached. The greater the visual angle, the more distinctly are the details of the object perceived. On the other See also:hand, as the observer recedes from the object, the apparent size, and also the image on the retina diminishes; details become more and more confused, and gradually, after a while, disappear altogether, and ultimately the See also:external configuration of the object as a whole is no longer recognizable. This See also:case arises when the visual angle, under which the object appears, is approximatel}, a See also:minute of arc; it is due to the physiological construction of the retina, for the ends of See also:nerve See also:fibres, which receive the impression of See also:light, have themselves a definite size. The See also:lower limit of the resolving power of the eye is reached when the distance is approximately 3438 times the size of the object. If the object be represented by two See also:separate points, these points would appear distinct to the normal eye only so See also:long as the distance between them is at the most only 3438 times smaller than their distance from the eye. When the latter distance is increased still further, the two appear as one. Therefore when it is desired to distinctly recognize exceedingly small objects or details of such, they are brought as near as possible to the eye. The eye is strained in bringing its focal length to the smallest possible amount, and when this See also:strain is long continued it may cause See also:pain. When the shortest distance obtained by the highest strain of accommodation is insufficient to recognize small objects, distinct vision is possible at even a shorter distance by placing a very small See also:diaphragm
between the eye and the object, the pencils of rays proceeding from the object-points, which otherwise are limited by the pupils of the eye, being thus restricted by the. diaphragm. The object is then projected with such acute pencils on the See also:plane focused for, in this case on the plane on which the eye can just accommodate itself, that the circle of confusion arising there is still so small that it is below the limit of angular visual distinctness and on that See also:account appears as a See also:sharp point. However, the loss of light in this See also:procedure is extraordinarily large, so that only most intensely illuminated objects can be investigated.
A naked See also:short-sighted eye, which would be corrected for distant objects by a spectacle See also:glass of —Io diopters, may approach the object up to about 4 in. and have a sharp image upon the retina without any strain whatever. For the observation of small objects, a myopic eye is consequently See also:superior to a normal eye; and the normal eye in its turn is superior to the hypermetropic one. When the details are no longer recognizable by the unaided eye, the magnifying glass or the simple microscope is necessary. As a See also:rule large magnification is not demanded from the former, but a larger See also: If y (fig. 2) be the object the image appears to a normal L Regulation of the Rays?--In using See also:optical See also:instruments the eye in See also:general is moved just as in See also:free vision; that is to say, the See also:attention is fixed upon the individual parts of the image one after another, the eye being turned in its cavity. In this case the eye is always directed so that the See also:part of the image which is wished to be viewed exactly falls upon the most sensitive portion of the retina, viz. the macula lutea (yellow spot). Corresponding to the size of the yellow spot only a small fraction of the image appears particularly distinctly. The other portions which are reproduced on the retina on the regions surrounding the yellow spot will also be perceived, but with reduced See also:definition. These external and less sensitive parts of the retina, therefore, merely give See also:information as to the general arrangement of the objects and to a certain extent See also:act as See also:guide-See also:post in See also:order to show quickly and conveniently, although not distinctly, the places in the image which should claim See also:special attention. Vision with a motionless eye, or " indirect vision," gives a general view over the whole object with particular definition of a small central portion. Vision with a movable eye, or See also:direct vision," gives exact information as to the parts of the object one after another. The simple microscope permits such vision. If the See also:instrument has a sensible lens See also:diameter, and is arranged so that the centre of rotation of the eye can coincide with the intersection of the See also:principal rays, the lens can then See also:form with the eye a centred See also:system. Such lenses are termed " lenses for direct vision." By moving the eye about its centre of rotation M the whole field can be examined. The margin of the See also:mount of the lens serves as the diaphragm of the field of view. The selection of the rays emerging from the lens and actually employed in forming the image is undertaken by the See also:pupil of the eye which, in this case, is consequently the exit pupil of the instrument. In fig. 3 P'P'1 designates the exit pupil of the L eye situated behind the system L with passive accommodation at a very great distance under the angle w'. Since H' P = F 0, = y, from the focal length of the simple microscope, the visual angle w' is given by tan w'/y=I/f'=V, (I) in which f', = H' F', is the image-See also:side focal length (see LENS). Since the lens is bounded by See also:air, the image- and object-side focal lengths f' and f are equal. The value 1/f' or V in (1), is termed the power of the lens. In most cases the number of " diameters " of the simple microscope is required; i.e. the ratio between the apparent sizes of the object when observed through the microscope and when viewed by the naked eye. When a person of normal vision views a small object, he brings it to the distance of distinct vision, which would See also:average about lo in. The apparent size is then (fig. I) tan w = y/l, where l = lo in., whilst the apparent size of the object viewed through the magnifying glass would result from the See also:formula (I) tan w' =y/f. Consequently the number of diameters will be N = tan w'/tan w = y/f . l/y = l/f =V.1; (2) it is thus equal to the magnifying power multiplied by the distance of distinct vision, or the number of times that the focal length is contained in io in. Since this value for the distance of distinct vision is only conventional, it is understood that the capacity of the simple micro-See also:scope given in (2) holds See also:good only for eyes accustomed to examine small objects to in. away; and observation through the magnifying glass must be undertaken by the normal eye with passive accommodation. A lens of I in. focal length must be spoken of, according to this notation, as a X to lens, and a lens of is in. focal length as a X too lens. Obviously the position of a normal eye free from accommodation is immaterial for determining the magnification. A X to magnification is, however, by no means guaranteed to a myopic eye of—to D by a lens of i in. See also:focus. Since this short-sighted observer can view the object with the naked eye with no inconvenience to himself at 4 in. distance, it follows (to him) the apparent size is tan w =y/4; and to secure convenient vision through the lens the short-sighted person would bring the object to such a distance that a virtual, magnified image would be projected in his punctum remotum. In addition it will be supposed that the centre of the pupil of the observer coincides with the back focal point of the system. The apparent size of the object seen through the lens is then tan w' = y/f. The magnification, resulting from the simple microscope of 1 in. focus, is here N=tan w'/tan w= y/f•4/y=4/f=4. Thus, while a lens of I in. focal length assures to the normal-sighted person a X to magnification, it affords to the short-sighted individual only X 4. On the other hand, it is even of greater use to the hypermetropic than to the observer of normal sight. From this it appears that each observer obtains specific advantages from one and the same simple microscope, and also the individual observer can obtain different magnifications by either using different accommodations, or by viewing in passive accommodation. vFIG. 3. lens, and the image of P'P'1, i.e. PP1, which is formed by the lens, limits the See also:aperture of the pencils of rays on the object-side; consequently it is the entrance pupil of the instrument. Since the exit pupil moves in observing the whole field, the entrance pupil also moves. The principal rays, which on the object-side connect the object-points with the centre of the entrance pupil, intersect the See also:axis on the image-side at the centre of rotation M of the eye. M is therefore the intersection of the principal rays. So long as the exit pupil is completely filled the brightness of the image will be approximately equal to that of free vision. If, however, we See also:fix the points lying towards the margin of the field of view, the diaphragm gradually cuts off more and more of the rays which were necessary to fill the pupil, and in consequence the brightness gradually fall's off to zero. This vignetting can be observed in all lenses. In most cases, and also in corrected systems, the intersection of the principal rays is no longer available for the centre of rotation of the eye, and this See also:kind of observation is impossible. In some instruments observation of the whole available field is only possible when the See also:head and eye are moved at the same See also:time, the lens retaining its position. Dr M. von Rohr terms this kind of vision " peep .hole observation." It has mainly to be considered in connexion with powerful magnifying glasses. In most cases a diaphragm regulates the rays. Fig. 4 shows the position of the diaphragms to be considered in this kind of observation. PP1 is the" entrance pupil, P'P1' the exit pupil, and GG the diaphragm. The inter-See also:section of the principal rays in this case lies in the See also:middle of the entrance pupil or of the exit pupil. By head and eye See also:motion FIG. 4. the various parts of the whole field can be viewed one after another. The distance of the eye from the lens is here immaterial. In this case also the See also:illumination must fall to zero by the vignetting of the pencils coming from objects at the margin of the field of view. C and D are the outermost rays which can pass through the instrument. Magnifying glasses are often used for viewing three-dimensional objects. Only points lying on the plane focused for can be sharply reproduced in the retina, which acts as object-plane to the retina. 1 See also LENS. All points lying out of this plane are reproduced as circles of See also:con-See also:fusion. The central See also:projection, of which the centre is the middle point of the entrance pupil on the plane focused for, will show in weaker systems, or those very much stopped down, a certain finite See also:depth of definition; that is to say, the totality of points, which See also:lie out of the plane focused for, and which are projected with circles of confusion so small that they appear to the eye as sharp points, will include the sharp object See also:relief, and determine the depth of definition of the lens. With increasing magnification the depth of definition diminishes, because the circles of confusion are greater in consequence of the shorter focal length. Very powerful simple microscopes have hardly any depth of definition so that in fact only points lying in one plane can be seen sharply with one focusing. Illumination.—So long as the pupil of the observer alone under-takes the regulation of the rays there is no perceptible diminution of illumination in comparison with the naked eye vision. The losses of light which occur in this case are due to reflection, which takes See also:place in the passage of the light through the glass surfaces. In a lens with two bounding surfaces in air there is a loss of about 9 %; and in a lens system consisting of two separated lenses, i.e. with four surfaces in air, about 17 %. Losses due to absorption are almost zero when the lenses are very thin, as with lenses of small diameter. A.very marked diminution in illumination occurs, however, when the exit pupil of the instrument is smaller than the pupil of the eye. In such instruments an arrangement is often required to intensely illuminate the object. Forms of the Simple Microscope.—If the See also:ordinary See also:convex lens be employed as magnifying glass, great aberrations occur even in See also:medium magnifications. These are: (1) See also:chromatic See also:aberration, (2) spherical aberration and (3) astigmatism (see ABERRATION). When the pupil regulates the aperture of the rays producing the image the aberrations of the ordinary lenses increase considerably with the magnification, or, what 'amounts to the same thing, with the increase in the curvature of the surfaces. For lenses of short focus the diameter of the pupil is too large, and diaphragms must be employed which strongly diminish the aperture of the pencils, and so reduce the errors, but with a falling off of illumination. To reduce the aberrations See also:Sir See also:David See also:Brewster proposed to employ in the place of glass transparent minerals of high refractive See also:index and See also:low See also:dispersion. In this manner lenses of short focus can be produced having lower curvatures than glass lenses necessitate. The See also:diamond has the requisite optical properties, its index of See also:refraction being about 1.6 times as large as that of ordinary glass. The spherical aberration of a diamond lens can be brought down to one-ninth of a glass lens of equal focus. Apart, however, from the cost of the See also:mineral and its very difficult working, a source of See also:error lies in its want of homogeneity, which often causes a See also:double or even a triple image. Similar attempts made by See also:Pritchard with sapphires were more successful. With this mineral also spherical and chromatic aberration are a fraction of that of a glass lens, but double refraction, which involves a doubling of the image, is fatal to its use. Improvements in glass lenses, however, have rendered further experiments with See also:precious stones unnecessary. The simplest was a See also:sphere of glass, the See also:equator of which (i.e. the mount) formed the diaphragm. See also:Wollaston altered this by taking two plano-convex lenses, placing the plane surfaces towards each other and employing a diaphragm between the two parts (fig 5). Wollaston. Brewster. Brewster (See also:Stanhope). Sir David Brewster found that Wollaston's form worked best when the two lenses were hemispheres and the central space was filled up with a transparent See also:cement having the same refractive index as the glass; he therefore used a sphere and provided it with a groove at the equator (see fig. 6). Coddington employed the same construction, and for this See also:reason this See also:device is frequently called the Coddington lens; although he brought the Wollaston-Brewster lens into general See also:notice, he was neither the inventor nor claimed to be. This lens reproduced all points of a concentric spherical See also:surface simultaneously sharp. A construction also employing one piece of glass forms the so-called Stanhope lens (fig. 7), which was really due to Brewster. This is a glass See also:cylinder, the two ends of which are spherical surfaces. The more strongly curved surface is placed next the eye, the other serves at the same time as specimen See also:carrier. This lens is employed in articles found in tourist resorts as a magnifying glass for See also:miniature photographs of the locality. Doublets, &c.—To remove the errors which the above lenses showed, particularly when very short focal lengths were in question, lens combinations were adopted. The individualcomponents required weaker curvatures and permitted of being more correctly manufactured, and, more particularly, the See also:advantage of reduced aberrations was the predominant, See also:factor.
Wollaston's doublet (fig. 8) is a See also:combination of two piano-convex lenses, the focal lengths of which are in the ratio of 3 : I ; the plane Wollaston. See also:Fraunhofer. See also: Similar doublets composed of two plano-convex lenses are the Fraunhofer (fig. 9) and the Wilson (fig. 1o). Axial aberration is reduced by distributing the refraction between two lenses; and by placing the two lenses farther apart the errors of the pencils of rays proceeding from points lying outside the axis are reduced. The Wilson has a greater distance between the lenses, and also a reduction of the chromatic difference of magnification, but compared with the Fraunhofer it is at a disadvantage with regard to the size of the free working distance, i.e. the distance of the object from the lens surface nearer it. By introducing a dispersive lens of See also:flint the magnifying glass could be corrected for both chromatic and spherical aberrations. See also:Browning's " platyscopic " lens and the Steinheil " aplanatic " lens (fig. 11) are of this type. Both yield a field of good definition free from See also:colour. The manner in which the eye uses such a lens was first effectively taken into account by M. von Rohr. These anastigmatic lenses, which are manufactured up to X 40, are chromatically and spherically corrected, and for a middle diaphragm the errors of lateral pencils, distortion, astigmatism and See also:coma are eliminated. " Peep-hole ' observation is employed, observation being made by moving the head and eye while the lens is held steady. Even in powerful magnifications a good image exists in all parts of a relatively large field, and the free working distance is fairly large. For especially large free working distances the corrections See also:pro-posed by Chevalier and carried out by E. Briicke must be noticed (fig. 12). To an achromatic collective lens, which is turned towards the object, a dispersive lens is combined (this type to a certain extent belongs to the See also:compound microscope). By altering the distance of the collective and dispersive members the magnification can be widely varied. Through the large free working distance, which for certain See also:work offers great advantages, the size of the field of view is diminished. In magnifying glasses for direct vision the eye must always be considered. The lens is brought as See also:close as possible to the eye so as to view as large a field as possible. The watchmaker's glass is one of the earliest forms of this kind. Gullstrand showed how to correct these lenses for direct vision, i.e. to eliminate distortion and astigmatism when the centre of rotation of the eye coincided with the point where the principal rays crossed the axis. Von Rohr fulfilled this See also:condition by constructing the Verant lens, which are low power systems intended for viewing a large See also:flat field. Stands.—For dissecting or examining objects it is an advantage to have both hands free. Where very short focus simple micro-scopes are employed, using high magnifications, it is imperative to employ a stand which permits exact focusing and the use of a special See also:illuminating apparatus. Since, however, only relatively low See also:powers are now employed, the ordinary See also:rack and pinion See also:movement for focusing suffices, and for illuminating the object only a See also:mirror below the See also:stage is required when the object is transparent, and a condensing lens above the stage when opaque.
Dissecting stands vary as to portability, the size of the stand, and the manner in which the See also:arm-rests are arranged. A stand is shown in fig. 57 (See also:Plate). On the heavy horseshoe See also:foot is a See also:column carrying the stage. In the column is the guide for the rack-and-pinion movement. Lenses of various magnifications can be adapted to the carrier and moved about over the stage. The rests can be attached to the stage, and when done with folded together. Illumination of transparent objects is effected by the universal-jointed mirror. By turning the knob A, placed at the front corner of
the stage, a See also:black or See also: When the recognition of the arrangement in space of small objects is desired a stereoscopic lens can be used. In most cases refracting and reflecting systems are arranged so that the natural interpupillary distance is reduced. Stereoscopic lenses can never be powerful systems, for the See also:main See also:idea is the recognition of the depth of objects, so that only systems having a sufficient depth of definition can be utilized. Very often such stereoscopic lenses, owing to faulty construction, give a false idea of space, ignoring the errors which are due to the alteration of the inter-pupillary distance and the visual angles belonging to the principal rays at the object-side (see See also:BINOCULAR INSTRUMENTS). Additional information and CommentsThere are no comments yet for this article.
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